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After washing, samples were dehydrated in a series of 50, 75, 90, and 100% tert-butyl alcohol (10 min each), followed by gradient de-ethanolization with 50, 75, 90, and 100% tert-butyl alcohol for 10 min, respectively, drying at the critical point, and vacuum plating

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Advertisement Article continues below this ad But Sly and the Family Stone still leaned into their East Bay roots with an album cover shoot atop the Oakland sculpture Mid-Century Monster. The band graduated to premier local venues like the Fillmore, where they played with the Grateful Dead, and the Fillmore East, where they opened for Jimi Hendrix

The global prevalence of bipolar disorder is estimated to be 0.5% and the associated disability-adjusted life years are 67 1